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11.
Liu T Qian WJ Gritsenko MA Xiao W Moldawer LL Kaushal A Monroe ME Varnum SM Moore RJ Purvine SO Maier RV Davis RW Tompkins RG Camp DG Smith RD;Inflammation the Host Response to Injury Large Scale Collaborative Research Programm 《Molecular & cellular proteomics : MCP》2006,5(10):1899-1913
Although human plasma represents an attractive sample for disease biomarker discovery, the extreme complexity and large dynamic range in protein concentrations present significant challenges for characterization, candidate biomarker discovery, and validation. Herein we describe a strategy that combines immunoaffinity subtraction and subsequent chemical fractionation based on cysteinyl peptide and N-glycopeptide captures with two-dimensional LC-MS/MS to increase the dynamic range of analysis for plasma. Application of this "divide-and-conquer" strategy to trauma patient plasma significantly improved the overall dynamic range of detection and resulted in confident identification of 22,267 unique peptides from four different peptide populations (cysteinyl peptides, non-cysteinyl peptides, N-glycopeptides, and non-glycopeptides) that covered 3,654 different proteins with 1,494 proteins identified by multiple peptides. Numerous low abundance proteins were identified, exemplified by 78 "classic" cytokines and cytokine receptors and by 136 human cell differentiation molecules. Additionally a total of 2,910 different N-glycopeptides that correspond to 662 N-glycoproteins and 1,553 N-glycosylation sites were identified. A panel of the proteins identified in this study is known to be involved in inflammation and immune responses. This study established an extensive reference protein database for trauma patients that provides a foundation for future high throughput quantitative plasma proteomic studies designed to elucidate the mechanisms that underlie systemic inflammatory responses. 相似文献
12.
Gritsenko VA Ivanov IuB Zhurlov OS 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2006,(4):16-19
Testing 54 strains of staphylococci (Staphylococcus aureus, S. epidermidis, S. haemolyticus, S. warneri, S. hominis, S. capitis) revealed that S. aureus in contrast to coagulase-negative staphylococci (CNS) is more resistant to bactoriocidal action of human thrombodefensins (resistance index: 60.3 vs 25.6%), less hydrophilicolipophilic balance-HLB: -0.42 vs -0.64) and less charged (x-potential: -32.4 vs -35.6 mV). In groups of staphylococci (S. aureus and CNS) correlation links of bacterial resistance to human thrombodefensins with their HLB and x-potential (r=-0.32...-0.36). By In vitro experiments, it was shown that 5 passages of staphylococci in meat-peptonic broth with human thrombodefensins (50 mkg protein/ml) lead to adaptation of bacteria followed by the formation of resistance to cationic peptides from thrombocytes, a decrease of hydrophobicity and x-potencial. The role of physico-chemical properties in providing thrombodefensin-resistance of staphylococci as a developmental factor of infectious-and-inflammatory process and persistence of bacteria was confirmed with Salmonella infection. 相似文献
13.
Golimbet VE Alfimova MV Gritsenko IK Ebshteĭn RP 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》2006,56(4):457-463
Dopamine neurotransmissin is thought to play a relevant role in behavioral reinforcement system. Polymorphism of the genes involved in dopamine system has been reported for association with psychological traits related to impulsive and sensation seeking behaviors. The study was aimed at a search for association of catechol-O-metyltransferase (COMT) and dopamine receptor D4 (DRD4) gene polymorphism with personality traits in Russian population. A sample comprised 130 subjects. It was found that carriers of the Met/Met COMT genotype had higher scores of novelty seeking as compared to those with the Val/Val and Met/Met genotypes. The association was observed in women only. In the presence of the C allele of the DRD4 gene, females with the Met/Met genotype demonstrated higher scores on extraversion and hypomania. The results are consistent with the current theoretical concepts on the regulation of dopamine neurotransmission in the brain. 相似文献
14.
Zhang W Culley DE Gritsenko MA Moore RJ Nie L Scholten JC Petritis K Strittmatter EF Camp DG Smith RD Brockman FJ 《Biochemical and biophysical research communications》2006,349(4):1412-1419
High efficiency capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to examine the proteins extracted from Desulfovibrio vulgaris cells across six treatment conditions. While our previous study provided a proteomic overview of the cellular metabolism based on proteins with known functions [W. Zhang, M.A. Gritsenko, R.J. Moore, D.E. Culley, L. Nie, K. Petritis, E.F. Strittmatter, D.G. Camp II, R.D. Smith, F.J. Brockman, A proteomic view of the metabolism in Desulfovibrio vulgaris determined by liquid chromatography coupled with tandem mass spectrometry, Proteomics 6 (2006) 4286-4299], this study describes the global detection and functional inference for hypothetical D. vulgaris proteins. Using criteria that a given peptide of a protein is identified from at least two out of three independent LC-MS/MS measurements and that for any protein at least two different peptides are identified among the three measurements, 129 open reading frames (ORFs) originally annotated as hypothetical proteins were found to encode expressed proteins. Functional inference for the conserved hypothetical proteins was performed by a combination of several non-homology based methods: genomic context analysis, phylogenomic profiling, and analysis of a combination of experimental information, including peptide detection in cells grown under specific culture conditions and cellular location of the proteins. Using this approach we were able to assign possible functions to 20 conserved hypothetical proteins. This study demonstrated that a combination of proteomics and bioinformatics methodologies can provide verification of the expression of hypothetical proteins and improve genome annotation. 相似文献
15.
O. V. Protasova I. A. Maksimova L. L. Klimenko E. V. Gritsenko M. N. Komarova 《Biology Bulletin》2002,29(3):216-220
We studied the content of Al, Cd, Co, Cu, Fe, Mg, Mn, P, Pb, S, and Zn in the liver, kidneys, lungs, and spleen of mice with total radiation dose of 7.5 Gy using atomic emission spectral analysis with an inductively coupled argon plasma. The qualitative content of macro- and microelements and coordination between their concentrations statistically determined from coefficients of linear correlation differ between the tissues of the irradiated and control animals. Radiation damage decreases phosphorus content in all studied organisms and is a marker of disturbed conjugated oxidative phosphorylation. The most significant radiation-induced disturbance of macro- and microelement balance was detected in the spleen; it features decreased content of phosphorus, magnesium, and cobalt, as well as increased content of zinc and aluminum and considerably increased iron content. The revealed macro- and microelements disbalance in spleen, liver, kidneys, and lungs can be considered as a test for primary biological response to radiation damage. 相似文献
16.
DNA duplexes with reactive dialdehyde groups as novel reagents for cross-linking to restriction- modification enzymes. 总被引:1,自引:1,他引:0 下载免费PDF全文
M G Brevnov O M Gritsenko S N Mikhailov E V Efimtseva B S Ermolinsky A Van Aerschot P Herdewijn A V Repyk E S Gromova 《Nucleic acids research》1997,25(16):3302-3309
To create new, effective reagents for affinity modification of restriction-modification (R-M) enzymes, a regioselective method for reactive dialdehyde group incorporation into oligonucleotides, based on insertion of a 1-beta-D-galactopyranosylthymine residue, has been developed. We synthesized DNA duplex analogs of the substrates of the Eco RII and Mva I R-M enzymes that contained a galactose or periodate-oxidized galactose residue as single substituents either in the center of the Eco RII (Mva I) recognition site or in the flanking nucleotide sequence. The dependence of binding, cleavage and methylation of these substrate analogs on the modified sugar location in the duplex was determined. Cross-linking of the reagents to the enzymes under different conditions was examined. M. Eco RII covalent attachment to periodate-oxidized substrate analogs proceeded in a specific way and to a large extent depended on the location of the reactive dialdehyde group in the substrate. The yield of covalent attachment to a DNA duplex with a dialdehyde group in the flanking sequence with Eco RII or Mva I methylases was 9-20% and did not exceed 4% for R. Eco RII. 相似文献
17.
Ham BM Yang F Jayachandran H Jaitly N Monroe ME Gritsenko MA Livesay EA Zhao R Purvine SO Orton D Adkins JN Camp DG Rossie S Smith RD 《Journal of proteome research》2008,7(6):2215-2221
Ongoing optimization of proteomic methodologies seeks to improve both the coverage and confidence of protein identifications. The optimization of sample preparation, inclusion of technical replicates (repeated instrumental analysis of the same sample), and biological replicates (multiple individual samples) are crucial in proteomic studies to avoid the pitfalls associated with single point analysis and under-sampling. Phosphopeptides were isolated from HeLa cells and analyzed by nano-reversed phase liquid chromatography electrospray ionization tandem mass spectrometry (nano-RP-LC-MS/MS). We observed that a detergent-based protein extraction approach, followed with additional steps for nucleic acid removal, provided a simple alternative to the broadly used Trizol extraction. The evaluation of four technical replicates demonstrated measurement reproducibility with low percent variance in peptide responses at approximately 3%, where additional peptide identifications were made with each added technical replicate. The inclusion of six technical replicates for moderately complex protein extracts (approximately 4000 uniquely identified peptides per data set) affords the optimal collection of peptide information. 相似文献
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